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Gene expression levels were determined by qRT-PCR in the Quantifast SYBR Green PCR mix (Qiagen GmbH, Germany) with a reaction condition of initial denaturation at 95 °C for 5 min and then 40 cycles of 95 °C for 10 s and 60 °C for 30 s in QuantStudio 3 machine

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Zinc deficiency aggravates oxidative stress leading to inflammation and fibrosis in lung of mice