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Taken together, the combined burden of on-target toxicity and off-target effects constrains the therapeutic window and constitutes a practical barrier to clinical development, making it essential to pair analog optimization with delivery strategies that confine exposure to lesions and minimize unintended pathway engagement in normal tissues

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CLSM images upon incubation of NP4-NR with the cancer cells showed the steadily increase of the fluorescence inside the cancer cells, reaching a maximal emission after incubation for 4 h